Rnaheat

# 2) Restrict the temperature range and step size
echo 'GGGAAAUCC' | RNAheat --Tmin 10 --Tmax 80 --stepsize 0.5 > heat.tsv

# 3) Model a circular RNA with G-quadruplex support
echo 'GGGAAAUCC' | RNAheat --circ --gquad > heat.tsv

Recommended Workflow

1. Prepare input RNA sequence(s) in plain text or FASTA-like format
2. Set temperature range with --Tmin and --Tmax (default 0–100°C) and adjust --stepsize as needed
3. Run RNAheat with appropriate options (e.g., --circ for circular RNA, --gquad for G-quadruplex)
4. Parse output pairs of temperature (°C) and specific heat (kcal/(mol*K)) for downstream analysis

Guardrails

• Output is tabular (temperature, specific heat) to stdout; redirect to file for persistence
• Input stops at a line containing only @ or EOF; ensure proper sequence delimiting
• Smoothing via -m/--ipoints affects curve shape; higher values produce smoother results at cost of resolution