Nanotoxicology skill for in vitro cytotoxicity assessment and cell viability analysis
name cytotoxicity-assay-analyzer description Nanotoxicology skill for in vitro cytotoxicity assessment and cell viability analysis allowed-tools ["Read","Write","Glob","Grep","Bash"] metadata {"specialization":"nanotechnology","domain":"science","category":"applications","priority":"high","phase":6,"tools-libraries":["Plate reader analysis software","Cell imaging analysis"]} Cytotoxicity Assay Analyzer Purpose The Cytotoxicity Assay Analyzer skill provides comprehensive in vitro toxicity assessment of nanomaterials, enabling systematic evaluation of cell viability, determination of safe exposure levels, and identification of toxicity mechanisms. Capabilities MTT/MTS/WST assay analysis Live/dead staining quantification IC50/EC50 calculation Dose-response curve fitting Cell morphology analysis Apoptosis/necrosis detection Usage Guidelines Cytotoxicity Assessment Assay Selection Choose appropriate viability assay Consider nanoparticle interference Include proper controls Data Analysis Calculate percent viability Fit dose-response curves Determine IC50/EC50 Result Interpretation Compare to benchmark materials Assess mechanism of toxicity Report with appropriate context Process Integration Nanomaterial Safety Assessment Pipeline Nanoparticle Drug Delivery System Development Input Schema { "nanomaterial" : "string" , "cell_line" : "string" , "assay_type" : "mtt|mts|wst|live_dead|ldh" , "concentrations" : [ "number" ] , "exposure_time" : "number (hours)" } Output Schema { "viability_data" : [ { "concentration" : "number" , "viability" : "number (%)" , "std_dev" : "number" } ] , "dose_response" : { "ic50" : "number" , "ic50_unit" : "string" , "hill_slope" : "number" , "r_squared" : "number" } , "toxicity_classification" : "string" , "mechanism_indicators" : [ "string" ] }