Use when aligning sequencing reads to a reference using Bowtie 2's large-index alignment engine.
bowtie2-align-l/home/vimalinx/miniforge3/envs/bio/bin/bowtie2-align-lbowtie2-align-l when your reference was indexed in Bowtie 2 large-index format (.bt2l), typically for very large references.bowtie2 wrapper.bowtie2 wrapper unless you need to force the large-index executable directly.# Align unpaired reads against a large Bowtie 2 index
bowtie2-align-l -x ref_large -U reads.fq -S aln.sam
# Align paired-end reads with multiple threads
bowtie2-align-l -x ref_large -1 reads_R1.fq -2 reads_R2.fq -p 8 -S aln.sam
# Use local alignment with a more sensitive preset
bowtie2-align-l -x ref_large -U reads.fq --very-sensitive-local -S aln.sam
# Report up to 5 alignments per read
bowtie2-align-l -x ref_large -U reads.fq -k 5 -S aln.sam
.bt2l files) exists for your reference genome-1/-2 (paired), -U (unpaired), --interleaved, or -b (BAM)--end-to-end or --local) and optionally a preset (e.g., --sensitive-local)-x <index>, input files, and -S <output.sam> (defaults to stdout).bt2l); Bowtie 1 indexes are not compatiblebowtie2 wrapper script instead of direct invocation as recommended by the tool warning-k or -a multi-hit reporting modes